Ozonated Olive Oil Test Report 1

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Consulting Clinical & Microbiological Laboratory, Inc.
333 S. W. 5th Ave. #620-7
Portland, OR 97204
Phone & FAX # 503-222-5279

Den (Zdenek) Rasplicka
Ozone Services - Yanco Industries Ltd. ¹
19889-53rd Avenue
Langley, B.C., V3A 6W2 CANADA

May 4, 1998

Dear Mr. Rasplicka:

This laboratory obtained the following fungi for you from the American Type Culture Collection on April 17, 1998:

• One fungus which causes Athlete's Foot = Trichophyton mentagrophytes
  Catalogue # 9129
• One fungus which causes Ringworm = Microsporum audouinii
  Catalogue # 26341
• Candida albicans
  Catalogue # 10231

Each of these cultures was spread on a Mueller Hinton Plate and some of the Ozonated Olive Oil (which you provided) was added to the surface of the media. The Plates were examined for areas of no growth around the Ozonated Olive Oil (000) after 3 days (72 hours) incubation. The distances of growth of each microorganisms from the edge of the Ozonated Olive Oil were:

T. mentagrophytes	M. audouinii	C. albicans
approximately 5 mm	approximately 5 mm	approximately 5 mm

The test was repeated and this time some Ozonated Olive Oil was rubbed in a circle approximately 30 mm in diameter on the Mueller Hinton Plate after the microorganisms had been spread over the surface. There was no or reduced growth on all of the Mueller Hinton Plates where the Ozonated Olive Oil was rubbed on the inoculated Mueller Hinton Plate after 3 days (72 hours) incubation.

T. mentagrophytes	M. audouinii	C. albicans
No growth	No growth	Reduced growth

The conclusion which can be drawn from this data is that Ozonated Olive Oil inhibits T. mentagrophytes, M. audouinii, and C. albicans by direct contact but does not diffuse into the media and inhibit growth.

It is conceivable that the Ozonated Olive Oil does not diffuse into the culture medium like many allopathic antimicrobial agents but it will inhibit the growth of microorganisms on the surface of the culture medium when rubbed onto the culture medium.

Do you want me to repeat the test again Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus using this technique of rubbing the Ozonated Olive Oil on the Mueller Hinton Plate rather than expecting the Ozonated Olive Oil to diffuse into the Mueller Hinton Culture medium?

Please advise if you would like any additional information.

Sincerely

J. P. Kilbourn, Ph. D.
Laboratory Director

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Footnotes

1. Ozone Services is now located at:
   390 Silver Queen Road,
   Burton, B.C.,
   V0G 1E0, Canada